Home

HPLC

Agilent 1100 系列 LC LC/MS 维护视频

Agilent 1100 化学工作站培训手册

HPLC使用过程中常见问题及解决方法 (1)  (2)

 

ITC 教程  ITC Tutorial  

 

配合物制备

      trans-EEcis-DDP, 15N trans-DDP

 

NMR核磁共振

 

NMR Frequency Table

 

Temperature Calibration

 

D2O溶剂中HDO信号随温度的变化

 

常用溶剂化学位移

[Link]  [Download – PDF]

 

一维氢谱的常规步骤

 

Protein Preparation: Isotope Labeling 

[Link]  [Download – PDF ]

 

 

Protein Selective Labeling

[Link]  [Download – PDF ]

 

 

Protein Preparation and NMR Sample Screening 

[Link]  [Download – PDF ]

 

 

NMR Sample Screening 

[Link]  [Download – PDF ]

 

 

Protein NMR Buffer Screening 

[Link]  [Download – PDF]

 

Protein Crystallization Buffer Screening

[Link] [Download – PDF]

 

 

NMR Structure Calculation

 

Automated NMR Structure Calculation With CYANA

[Link]  [Download – PDF]

 

 

实验方法

Precipitation of DNA by ethanol

 

Protein Desalting with Sephadex G25

 

Oligo DNA Concentration Measurement

 

Intein活性检测(体外GFP法)

 

 

常用数据

生物缓冲液pH范围

氨基酸溶解度

氨基酸化学位移

Oligonucleotide Properties Calculator

 

 

Miscellaneous

英文杂志缩写

 

 

Liu_Lab